MicroRNAs are small non-coding RNAs that regulate gene expression by inhibiting translation or
inducting degradation of the targeted mRNAs. Recent studies suggest that microRNA control
angiogenesis, however, the involvement of microRNAs in vessel maturation has not been fully
elucidated. Here, we propose to determine whether shear stress-induced endothelial microRNAs control
the interaction of ECs with pericytes. Specifically, we plan to study the regulation of endothelial-pericyte
interaction in vitro and vessel maturation in vivo by the shear stress-induced microRNAs miR-27a/b and
the miR-30 family and their predicted targets namely semaphorins and angiopoietin-2.